FAQ:
Precautions for the use of ultrasonic grinder?
1. The depth of the ultrasonic probe of the ultrasonic pulverizer into the water is about 1.5cm, and the height of the liquid
level is above 30mm. The probe must be centered and not attached to the wall. Ultrasound is a vertical longitudinal wave, and it
is not easy to form convection if inserted too deep, which will directly affect the crushing efficiency.
2. Remember to empty super. this point is very important. The longer the ultrasonic cell pulverizer air supersonic time, the
greater the damage to the instrument.
3. In order to prevent the liquid level from dropping after the ice bath melts and causing no load, the sample should be placed in a 1.5ml EP tube. Remember to fix the EP tube.
4. Ultrasonic parameter setting of ultrasonic pulverizer: set the working parameters of the instrument. For samples that are sensitive to temperature (such as bacteria), ice bath is generally used outside. The actual temperature must be lower than 25 degrees, and protein nucleic acid will definitely not be denatured. .
A. Time: The ultrasound time should not exceed 5 seconds each time, and the gap time should be greater than or equal to the ultrasound time to facilitate heat dissipation. The time setting should be based on the principle of short ultrasound time and multiple ultrasound times, which can extend the life of the ultrasound machine and probe.
B. Ultrasonic power of the ultrasonic pulverizer: it should not be too large to avoid splashing or foaming of the sample. If the sample capacity is less than 10ml, the power should be within 200W. Use a 2mm ultrasonic probe and turn the horn selection switch on the back of the panel to the corresponding Block; 10-200ml sample capacity power is between 200-400W, 6mm ultrasonic probe is used, and the horn behind the panel is set to the corresponding block; sample capacity power of 200ml or more is between 300-600W, and 10mm ultrasonic probe is selected. In addition, hit the horn behind the panel to the corresponding gear (the power of the small probe of 2mm is strictly prohibited to exceed 350W).
C. The container of the ultrasonic pulverizer: choose the beaker as large as there are samples, which is beneficial to the convection of the samples in the ultrasonic and improves the crushing efficiency. For example, use a 20mL beaker for a 20mL treatment volume.
5. The maintenance method of the ultrasonic grinder: After use, scrub the probe with alcohol or use clean water for ultrasonic cleaning.
6. Before using the ultrasonic pulverizer, the ultrasonic horn must be inserted into the sample before it can be turned
on. No-load operation is not allowed.
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